protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.Mass spectrophotometric characterization of proteins and peptidesN-terminal sequencing of proteins with blocked N terminiInternal amino acid sequence analysis after protease digestion in-gel and on-blotImproved microscale peptide purification methodsComputer analysis of protein sequencesNew protocols tested and refined through everyday use in authors' laboratoriesUpdated reference chapter covering all aspects of protein microsequencing

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